Mirus Bio has developed the TransIT® Transfection Reagents for the delivery of all types of nucleic acids including DNA, siRNA, mRNA, viral RNA and oligonucleotides to mammalian cells. Each of the TransIT® Transfection Reagents exhibits low cellular toxicity enabling the acquisition of physiologically relevant data.
Additional significant benefits over competing products include:
· High Efficiency Transfection—Independent of the type of nucleic acid being transfected, all the TransIT® Reagents promote high efficiency transfection, increasing experimental success.
· Low Cellular Toxicity—The proprietary formulations and the serum compatibility of the TransIT® Reagents promotes the maintenance of cell density and health, reducing potential experimental biases due to reagent toxicity.
· Easy to Use—The TransIT® protocols are simple with easy optimization which means you spend less time optimizing and more time performing the critical transfection experiments.
· Serum Compatibility—All the TransIT® transfection reagents transfect cells growing in the presence of serum, both simplifying the transfection procedure, and helping maintain cells under normal growth conditions.
· Proprietary Formulations—Developed and manufactured by Mirus Bio scientists.
#Label IT® Labeling Reagents
The LabelIT® Technology is a non-enzymatic chemical labeling method using an efficient one-step chemical reaction and facilitates the direct covalent attachment of non-radioactive reporter molecules and functional groups to DNA and RNA.
Special features:
· Directly label native and synthetic RNA and DNA of any length from any species
· Eliminates enzymatic incorporation and replication bias
· Fast and simple reaction
· Cost effective—save up to 50% per reaction compared to enzymatic methods
# In Vivo Delivery
The primary delivery and expression site using the hydrodynamic tail vein (HTV) delivery method is to the liver. The rapid injection into a rodent’s tail vein with a sufficient volume of nucleic acid solution used to elevate the pressure within the blood vessel and enhance the vessel’s permeability, thereby enabling passage of large nucleic acid molecules to target cells outside the blood vessel. It is detectable, but significantly less delivery to other organs including the spleen, lung, heart and kidneys is also possible. The advantages of using this method are efficacy, ease-of-use, and have ability to allow long-term gene expression.
